Product Description |
Recombinant Human GCGR/Glucagon receptor Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ala26-Lys136. |
Product Background |
The pancreatic hormone glucagon activates the glucagon receptor (GCGR), a class B seven-transmembrane G protein-coupled receptor that couples to the stimulatory heterotrimeric G protein and provokes PKA-dependent signaling cascades vital to hepatic glucose metabolism and islet insulin secretion. The GCGR expressed at the plasma membrane is constitutively ubiquitinated and upon agonist-activation, internalized GCGRs are deubiquitinated at early endosomes and recycled via Rab4-containing vesicles. |
Product Category |
Recombinant Protein |
Target |
GCGR/Glucagon receptor |
Synonyms |
Glucagon receptor; GL-R; GCGR; Glucagon R |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala26-Lys136 |
Molecular Weight |
The protein has a predicted MW of 15.96 kDa. Due to glycosylation, the protein migrates to 30-50 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human GCGR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human GCGR is greater than 95% as determined by SEC-HPLC. |
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