Product Description |
Recombinant Human Her2/ErbB2 Domain 4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Pro489-Cys630. |
Product Background |
ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. |
Product Category |
Recombinant Protein |
Target |
Her2/ErbB2 Domain 4 |
Synonyms |
CD340; EGFR2; ErbB2; HER2; HER-2; herstatin; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; NGL; p185erbB2; TKR1; ERBB2; HER-2/neu; NEU; ENV; ENVW; ERVWE1; HERV-7q; HERV-W-ENV; HERV7Q; HERVW; HERVWENV |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Pro489-Cys630 |
Molecular Weight |
The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Her2 Domain 4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human Her2 Domain 4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA. |
|
|
Human Her2 Domain 4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human Her2 Domain 4 is greater than 95% as determined by SEC-HPLC. |
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