Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein (MHC-HE010)

Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 43.4ng/ml determined by ELISA.

Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc-Avi Tag on ProA-Biosensor can bind Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag with an affinity constant of 53.70 nM as determined in BLI assay .

The purity of Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 43.4ng/ml determined by ELISA.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and RMFPNAPYL peptide

Catalog #MHC-HE010

Price

$341⁰⁰

Size

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Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein (MHC-HE010) Summary

Product Description

Recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and RMFPNAPYL peptide.

Product Background

The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of -the MHC Class I, B2M and RMFPNAPYL peptide of -the WT-1.

HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Specifications

Protein	HLA-A*02:01&B2M&WT-1 (RMFPNAPYL)
Synonyms	MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33;WIT2
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and RMFPNAPYL peptide
Molecular Weight	The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Activity	Immobilized Human HLA-A02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 43.4ng/ml determined by ELISA.
Product Tag	C-His-Avi
Fluorophore	Unconjugated
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human HLA-A02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 43.4ng/ml determined by ELISA.
	Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-HLA-A02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc-Avi Tag on ProA-Biosensor can bind Human HLA-A02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag with an affinity constant of 53.70 nM as determined in BLI assay .
	The purity of Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer is greater than 95% as determined by SEC-HPLC.