Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein (MHC-HE010)
Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein (MHC-HE010)
Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein (MHC-HE010)
  • Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and RMFPNAPYL peptide
Catalog #MHC-HE010
Regular price $310.00
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Product Description

Recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and RMFPNAPYL peptide.

Product Background

The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of -the MHC Class I, B2M and RMFPNAPYL peptide of -the WT-1.

Product Category

Recombinant Protein / Antibody discovery / MHC

Protein

HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer

Synonyms

MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33

MHC Form

Monomer

Allele

HLA-A*02:01

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and RMFPNAPYL peptide

Molecular Weight

The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.

Activity

Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

E.coli

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in 20mM Tris, 300mM NaCl, 10%Glycerol (pH 8.0).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA. Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA.
Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer is greater than 95% as determined by SEC-HPLC.

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