Human Hsp27/HSPB1-Protein (HSP-HE001)

Express System: E.coli
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Met1-Lys205

Catalog #HSP-HE001

Price

$330⁰⁰

Size

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Human Hsp27/HSPB1-Protein (HSP-HE001) Summary

Product Description

Recombinant Human Hsp27/HSPB1-Protein is expressed from E.coli with His tag at the C-Terminus.It contains Met1-Lys205.

Product Background

Heat shock protein beta-1 (HSPB1, also known as HSP27) is a small heat shock protein involved in many cellular processes and reportedly protects cells against oxidative stress. This protein is expressed only in insulin-dependent tissues (heart, skeletal muscle, and fat tissue), and expression of HspB7 is regulated by many different factors. HspB7 has an unusual N-terminal sequence, a conservative α-crystallin domain, and very short C-terminal domain lacking conservative IPV tripeptide involved in a small heat shock proteins oligomer formation.

Human Hsp27/HSPB1-Protein (HSP-HE001) Specifications/Details

Protein	Hsp27/HSPB1
Synonyms	Hsp27; HSPB1; HSP 27; SRP27; HSP28; CMT2F; HMN2B;
Accession	NP_001531.1
Species	Human
Biotinylated	no
Amino Acid Range	Met1-Lys205
Molecular Weight	The protein has a predicted MW of 23.74 kDa. The protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 2mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human Hsp27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Hsp27 is greater than 95% as determined by SEC-HPLC.