Human Hsp27/HSPB1-Protein (HSP-HE001)

  • Express System: E.coli
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Exact Sequence: Met1-Lys205

Catalog: HSP-HE001

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Product Description Recombinant Human Hsp27/HSPB1-Protein is expressed from E.coli with His tag at the C-Terminus.It contains Met1-Lys205.

Product Background

Heat shock protein beta-1 (HSPB1, also known as HSP27) is a small heat shock protein involved in many cellular processes and reportedly protects cells against oxidative stress. This protein is expressed only in insulin-dependent tissues (heart, skeletal muscle, and fat tissue), and expression of HspB7 is regulated by many different factors. HspB7 has an unusual N-terminal sequence, a conservative α-crystallin domain, and very short C-terminal domain lacking conservative IPV tripeptide involved in a small heat shock proteins oligomer formation.

Product Category

Recombinant Protein

Target

Hsp27/HSPB1

Gene/Protein ID

NP_001531.1

Species

Human

Exact Sequence

Met1-Lys205
Molecular Weight The protein has a predicted MW of 23.74 kDa. The protein migrates to 25-30 kDa based on Tris-Bis PAGE result.

Product Tag

C-His

Express System

E.coli
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS, 2mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Human Hsp27 is greater than 95% as determined by SEC-HPLC.
Human Hsp27 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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