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Human Hsp27/HSPB1-Protein (HSP-HE001) Summary
Product Description |
Recombinant Human Hsp27/HSPB1-Protein is expressed from E.coli with His tag at the C-Terminus.It contains Met1-Lys205. |
Product Background |
Heat shock protein beta-1 (HSPB1, also known as HSP27) is a small heat shock protein involved in many cellular processes and reportedly protects cells against oxidative stress. This protein is expressed only in insulin-dependent tissues (heart, skeletal muscle, and fat tissue), and expression of HspB7 is regulated by many different factors. HspB7 has an unusual N-terminal sequence, a conservative α-crystallin domain, and very short C-terminal domain lacking conservative IPV tripeptide involved in a small heat shock proteins oligomer formation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Hsp27/HSPB1-Protein (HSP-HE001) Specifications/Details
Protein |
Hsp27/HSPB1 |
Synonyms |
Hsp27; HSPB1; HSP 27; SRP27; HSP28; CMT2F; HMN2B; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Lys205 |
Molecular Weight |
The protein has a predicted MW of 23.74 kDa. The protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS, 2mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human Hsp27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human Hsp27 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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