Human Hsp27/HSPB1-Protein (HSP-HE001)
Human Hsp27/HSPB1-Protein (HSP-HE001)
  • Express System: E.coli
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Met1-Lys205
Catalog #HSP-HE001
Regular price $300.00
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Product Description
Recombinant Human Hsp27/HSPB1-Protein is expressed from E.coli with His tag at the C-Terminus.It contains Met1-Lys205.
Product Background
Heat shock protein beta-1 (HSPB1, also known as HSP27) is a small heat shock protein involved in many cellular processes and reportedly protects cells against oxidative stress. This protein is expressed only in insulin-dependent tissues (heart, skeletal muscle, and fat tissue), and expression of HspB7 is regulated by many different factors. HspB7 has an unusual N-terminal sequence, a conservative α-crystallin domain, and very short C-terminal domain lacking conservative IPV tripeptide involved in a small heat shock proteins oligomer formation.
Product Category
Recombinant Protein
Target
Hsp27/HSPB1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Met1-Lys205
Molecular Weight
The protein has a predicted MW of 23.74 kDa. The protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Product Tag
C-His
Express System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS, 2mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Hsp27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Hsp27 is greater than 95% as determined by SEC-HPLC.

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