Human IL-3 Protein (IL3-HE401)
Human IL-3 Protein (IL3-HE401)
Human IL-3 Protein (IL3-HE401)
Human IL-3 Protein (IL3-HE401)
  • Express System: E.coli
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ala20-Phe152
Catalog #IL3-HE401
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Product Description
 Recombinant Human IL-3 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Ala20-Phe152.
Product Background
 Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Product Category
 Recombinant Protein
Target
 IL-3
Synonyms
 IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala20-Phe152
Molecular Weight
 The protein has a predicted MW of 16.9 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human IL-3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 N-His-Avi
Express System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA (QC Test).
Human IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is < 0.2 ng/mL.
The purity of Human IL-3 is greater than 95% as determined by SEC-HPLC.
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