Human IL-3 Protein (IL3-HE401)

Express System: E.coli
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Ala20-Phe152

Catalog: IL3-HE401

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Product Description	Recombinant Human IL-3 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Ala20-Phe152.
Product Background	Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Product Category	Recombinant Protein

Target	IL-3
Synonyms	IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF
Gene/Protein ID	P08700
Species	Human
Exact Sequence	Ala20-Phe152
Molecular Weight	The protein has a predicted MW of 16.9 kDa same as Tris-Bis PAGE result.
Activity	Immobilized Human IL-3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-His-Avi
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human IL-3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA (QC Test).
	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is < 0.2 ng/mL.
	The purity of Human IL-3 is greater than 95% as determined by SEC-HPLC.
	Human IL-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.