Human IL-3 Protein (IL3-HE401)
Human IL-3 Protein (IL3-HE401)
  • Express System: E.coli
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ala20-Phe152
Catalog #IL3-HE401
Regular price $240.00
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Product Description
Recombinant Human IL-3 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Ala20-Phe152.
Product Background
Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Product Category
Recombinant Protein
Target
IL-3
Synonyms
IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ala20-Phe152
Molecular Weight
The protein has a predicted MW of 16.9 kDa same as Bis-Tris PAGE result.
Activity
Immobilized Human IL-3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
N-His-Avi
Express System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA (QC Test).
Human IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is < 0.2 ng/mL.
The purity of Human IL-3 is greater than 95% as determined by SEC-HPLC.
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