Name: Human IL-3 Protein (IL3-HE401)
Brand: KactusBio
SKU: IL3-HE401-100UG
Price: 264.0 USD
Availability: InStock

Express System: E.coli
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ala20-Phe152

Catalog #IL3-HE401

Size

100UG

500UG

Order now, ships by:

Product Description	Recombinant Human IL-3 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Ala20-Phe152.
Product Background	Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	IL-3
Synonyms	IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF
Accession	P08700
Species	Human
Biotinylated	no
Amino Acid Range	Ala20-Phe152
Molecular Weight	The protein has a predicted MW of 16.9 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human IL-3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-His-Avi
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human IL-3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA (QC Test).
	Human IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is < 0.2 ng/mL.
	The purity of Human IL-3 is greater than 95% as determined by SEC-HPLC.