Human IL-3 Protein (IL3-HE401)

  • Express System: E.coli
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human IL-3, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.61μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala20-Phe152

Catalog: IL3-HE401

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Product Description Recombinant Human IL-3 Protein is expressed from E.coli with His tag and Avi tag at the N-terminal.; It contains Ala20-Phe152.
Product Category Recombinant Protein
Target IL-3
Synonyms IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF
Gene/Protein ID P08700
Species Human
Exact Sequence Ala20-Phe152
Molecular Weight The protein has a predicted MW of 16.9 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human IL-3, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.61μg/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi
Express System E.coli
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-3, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.61μg/ml determined by ELISA.
The purity of Human IL-3 is greater than 95% as determined by SEC-HPLC.
Human IL-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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