Human IL-3 R alpha/CD123 Protein (IL3-HM2RA)
|Recombinant Human IL-3 R alpha/CD123 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Thr19-Arg305.
|Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily.The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias.
|IL-3 R alpha/CD123
|CD123; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL3RAY; IL3RX; IL3RY; MGC34174; IL-3 R alpha
|The protein has a predicted MW of 59.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
|Immobilized Human IL-3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA. See testing image for detail.
|> 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
|Less than 1EU per ug by the LAL method.
|Shipped at ambient temperature.
|Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
|Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
|Stability And Storage
|Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.