Human IL-3 R alpha/CD123 Protein (IL3-HM2RA)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human IL-3, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.61μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Thr19-Arg305

Catalog: IL3-HM2RA

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Product Description Recombinant Human IL-3 R alpha/CD123 Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Thr19-Arg305.
Product Category Recombinant Protein
Target IL-3 R alpha/CD123
Synonyms CD123; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL3RAY; IL3RX; IL3RY; MGC34174; IL-3 R alpha
Gene/Protein ID P26951-1
Species Human
Exact Sequence Thr19-Arg305
Molecular Weight The protein has a predicted MW of 59.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-3, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.61μg/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-3, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.61μg/ml determined by ELISA.
The purity of Human IL-3 R alpha is greater than 95% as determined by SEC-HPLC.
Human IL-3 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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