Human IL-5 Protein (IL5-HM401)
Catalog: IL5-HM401
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Product Description | Recombinant Human IL-5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ile20-Ser134. |
Product Background |
IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA). |
Product Category |
Recombinant Protein |
Target |
IL-5 |
Synonyms | Interleukin-5; IL-5; T-cell replacing factor; TRF; EDF |
Gene/Protein ID |
P05113 |
Species |
Human |
Exact Sequence |
Ile20-Ser134 |
Molecular Weight | The protein has a predicted MW of 16.1 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human IL-5, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA (QC Test). The affinity constant of 4.9nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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