Human IL-5 Protein (IL5-HM401)

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-5, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA (QC Test). The affinity constant of 4.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ile20-Ser134

Catalog: IL5-HM401

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Product Description	Recombinant Human IL-5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ile20-Ser134.
Product Background	IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA).
Product Category	Recombinant Protein

Target	IL-5
Synonyms	Interleukin-5; IL-5; T-cell replacing factor; TRF; EDF
Gene/Protein ID	P05113
Species	Human
Exact Sequence	Ile20-Ser134
Molecular Weight	The protein has a predicted MW of 16.1 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-5, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA (QC Test). The affinity constant of 4.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-5, His Tag immobilized on CM5 Chip can bind Human IL-5Ra, His Tag with an affinity constant of 4.9nM as determined in a SPR assay (Biacore T200).
	Immobilized Human IL-5, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA (QC Test).
	The purity of Human IL-5 is greater than 95% as determined by SEC-HPLC.
	Human IL-5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.