Name: Human M-CSF/CSF-1 Protein (CSF-HM401)
Brand: KactusBio
SKU: CSF-HM401-100UG
Price: 412.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human M-CSF, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Glu33-Asn190

Catalog #CSF-HM401

Size

100UG

500UG

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Product Description	Recombinant Human M-CSF/CSF-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu33-Asn190.
Product Background	CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.
Product Category	Recombinant Protein / Antibody discovery

Protein	M-CSF/CSF-1
Synonyms	CSF1; CSF-1; MCSF; M-CSF; MGC31930; Lanimostim
Accession	P09603-1
Species	Human
Biotinylated	no
Amino Acid Range	Glu33-Asn190
Molecular Weight	The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 28-34 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human M-CSF, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human M-CSF, His Tag captured on CM5 Chip via Anti-his Antibody can bind Human M-CSF R, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
	Immobilized Human M-CSF, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA (QC Test).
	Human M-CSF on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	Measured in a cell proliferation assay using RAW264.7 cells.The ED50 for this effect is 2.5 - 7.0 ng/mL.
	The purity of Human M-CSF is greater than 95% as determined by SEC-HPLC.