Human M-CSF/CSF-1 Protein (CSF-HM401)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human M-CSF, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu33-Asn190

Catalog: CSF-HM401

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Product Description Recombinant Human M-CSF/CSF-1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Glu33-Asn190.
Product Category Recombinant Protein
Target M-CSF/CSF-1
Synonyms CSF1; CSF-1; MCSF; M-CSF; MGC31930; Lanimostim
Gene/Protein ID P09603-1
Species Human
Exact Sequence Glu33-Asn190
Molecular Weight The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 28-34 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human M-CSF, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human M-CSF, His Tag captured on CM5 Chip via Anti-his Antibody can bind Human M-CSF R, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Immobilized Human M-CSF, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
The purity of Human M-CSF is greater than 95% as determined by SEC-HPLC.
Human M-CSF on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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