Human NKp30/NCR3/CD337 Protein (NKP-HM430)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NKp30, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. The affinity constant of 0.292 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu19-Thr138

Catalog: NKP-HM430

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Product Description Recombinant Human NKp30/NCR3/CD337 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu19-Thr138.

Product Background

NKp30, along with NKp44 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors". These receptors play a major role in triggering NK-mediated killing of most tumor cells lines.NKp30 stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation.

Product Category

Recombinant Protein

Target

NKp30/NCR3/CD337
Synonyms CD337; LY117; NCR3; NKp30; 1C7; MALS
Accession

Species

Human

Amino Acid Range

Leu19-Thr138
Molecular Weight The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 26-40 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human NKp30, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. The affinity constant of 0.292 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Human NKp30, His Tag with an affinity constant of 0.292 uM as determined in SPR assay (Biacore T200).
Immobilized Human NKp30, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
Immobilized Human NKp30, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H6, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test).
The purity of Human NKp30 is greater than 95% as determined by SEC-HPLC.
Human NKp30 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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