Product Description |
Recombinant Human NKp30/NCR3/CD337 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu19-Thr138. |
Product Background |
NKp30, along with NKp44 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors". These receptors play a major role in triggering NK-mediated killing of most tumor cells lines.NKp30 stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation. |
Product Category |
Recombinant Protein |
Protein |
NKp30/NCR3/CD337 |
Synonyms |
CD337; LY117; NCR3; NKp30; 1C7; MALS |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu19-Thr138 |
Molecular Weight |
The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 26-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human NKp30, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. The affinity constant of 0.292 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Human NKp30, His Tag with an affinity constant of 0.292 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human NKp30, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. |
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Immobilized Human NKp30, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H6, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test). |
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Human NKp30 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of Human NKp30 is greater than 95% as determined by SEC-HPLC. |
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