Human Transferrin R/CD71 Protein (TFR-HM401)

  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Transferrin R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test). The affinity constant of 27.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Cys89-Phe760

Catalog: TFR-HM401

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Product Description Recombinant Human Transferrin R/CD71 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Cys89-Phe760.

Product Background

The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.

Product Category

Recombinant Protein

Target

Transferrin R/CD71
Synonyms TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR

Gene/Protein ID

P02786

Species

Human

Exact Sequence

Cys89-Phe760
Molecular Weight The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 78-80 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Transferrin R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test). The affinity constant of 27.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Transferrin, His Tag immobilized on CM5 Chip can bind Human Transferrin R, His Tag with an affinity constant of 27.50 nM as determined in SPR assay (Biacore T200).
Immobilized Human Transferrin R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test).
The purity of Human Transferrin R is greater than 95% as determined by SEC-HPLC.
Human Transferrin R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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