Human Transferrin R/CD71 Protein (TFR-HM401)

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Transferrin R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA. The affinity constant of 27.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Cys89-Phe760

Catalog: TFR-HM401

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human Transferrin R/CD71 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Cys89-Phe760.
Product Category	Recombinant Protein

Target	Transferrin R/CD71
Synonyms	TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
Gene/Protein ID	P02786
Species	Human
Exact Sequence	Cys89-Phe760
Molecular Weight	The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 78-80 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Transferrin R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA. The affinity constant of 27.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human Transferrin, His Tag immobilized on CM5 Chip can bind Human Transferrin R, His Tag with an affinity constant of 27.50 nM as determined in SPR assay (Biacore T200).
	Immobilized Human Transferrin R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA.
	The purity of Human Transferrin R is greater than 95% as determined by SEC-HPLC.
	Human Transferrin R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.