Product Description |
Recombinant Human Transferrin R/CD71 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Cys89-Phe760. |
Product Background |
The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
Transferrin R/CD71 |
Synonyms |
TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Cys89-Phe760 |
Molecular Weight |
The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 78-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Transferrin R, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 9.2 ng/ml determined by ELISA (QC Test). The affinity constant of 11.26 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His-Avi |
Expression System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Human Transferrin, His Tag immobilized on CM5 Chip can bind Human Transferrin R, His Tag with an affinity constant of 11.26 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human Transferrin R, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 9.2 ng/ml determined by ELISA (QC Test). |
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Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Transferrin R is greater than 95% as determined by SEC-HPLC. |
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