Name: Human Transferrin R/CD71 Protein (TFR-HM401)
Brand: KactusBio
SKU: TFR-HM401-100UG
Price: 297.0 USD
Availability: InStock

Human Transferrin R/CD71 Protein (TFR-HM401)

Human Transferrin, His Tag immobilized on CM5 Chip can bind Human Transferrin R, His Tag with an affinity constant of 11.26 nM as determined in SPR assay (Biacore T200).

Immobilized Human Transferrin R, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 9.2 ng/ml determined by ELISA (QC Test).

Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Transferrin R is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi
Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Activity: Immobilized Human Transferrin R, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 9.2 ng/ml determined by ELISA (QC Test). The affinity constant of 11.26 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Cys89-Phe760

Catalog #TFR-HM401

Size

100UG

500UG

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Product Description	Recombinant Human Transferrin R/CD71 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Cys89-Phe760.
Product Background	The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
Product Category	Recombinant Protein / Antibody discovery

Protein	Transferrin R/CD71
Synonyms	TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
Accession	P02786
Species	Human
Biotinylated	no
Amino Acid Range	Cys89-Phe760
Molecular Weight	The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 78-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Transferrin R, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 9.2 ng/ml determined by ELISA (QC Test). The affinity constant of 11.26 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Avi
Expression System	HEK293
Purity	>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human Transferrin, His Tag immobilized on CM5 Chip can bind Human Transferrin R, His Tag with an affinity constant of 11.26 nM as determined in SPR assay (Biacore T200).
	Immobilized Human Transferrin R, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 9.2 ng/ml determined by ELISA (QC Test).
	Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Transferrin R is greater than 95% as determined by SEC-HPLC.