Human Transferrin R/CD71 Protein (TFR-HM401)
Human Transferrin R/CD71 Protein (TFR-HM401)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Transferrin R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Cys89-Phe760
Catalog #TFR-HM401
Regular price $270.00
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Product Description
Recombinant Human Transferrin R/CD71 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Cys89-Phe760.
Product Background
The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
Product Category
Recombinant Protein
Target
Transferrin R/CD71
Synonyms
TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Cys89-Phe760
Molecular Weight
The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 78-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Transferrin R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
N-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Transferrin R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test).
Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Transferrin R is greater than 95% as determined by SEC-HPLC.
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