Human TSLP (R127A, R130A) Protein (TSP-HM402)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TSLP (R127A, R130A) , His Tag at 5ug/ml (100ul/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Tyr29-Gln159(R127A,R130A)

Catalog: TSP-HM402

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Product Description	Recombinant Human TSLP (R127A, R130A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr29-Gln159(R127A,R130A).
Product Background	Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
Product Category	Recombinant Protein

Target	TSLP (R127A, R130A)
Synonyms	thymic stromal lymphopoietin; TSLP
Gene/Protein ID	Q969D9-1
Species	Human
Exact Sequence	Tyr29-Gln159(R127A,R130A)
Molecular Weight	The protein has a predicted MW of 17.7 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human TSLP (R127A, R130A) , His Tag at 5ug/ml (100ul/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human TSLP (R127A, R130A) , His Tag at 5ug/ml (100ul/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test).
	Immobilized Human TSLP (R127A,R130A) , His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-Human TSLP Ab., hFc Tag with the EC50 of 62.6ng/ml determined by ELISA.
	The purity of-Human TSLP (R127A, R130A) is greater than 95% as determined by SEC-HPLC.
	Human TSLP (R127A, R130A) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.