Product Description |
Recombinant Mouse APOA1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp25-Gln264. |
Product Background |
Apolipoprotein A1 (ApoA1) is a main protein moiety in high-density lipoprotein (HDL) particles. Generally, ApoA1 and HDL are considered as atheroprotective. In prooxidant and inflammatory microenvironment in the vicinity to the atherosclerotic lesion, ApoA1/HDL are subjected to modification. The chemical modifications such as oxidation, nitration, etc result in altering native architecture of ApoA1 toward dysfunctionality and abnormality. |
Product Category |
Recombinant Protein |
Target |
APOA1 |
Synonyms |
Apo-AI; ProapoA-I; Apoa1; Apolipoprotein A-I; APOA1; MGC117399; Alp-1; Brp-14; Ltw-1; Lvtw-1; Sep-1; Sep-2 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Asp25-Gln264 |
Molecular Weight |
The protein has a predicted MW of 54.7 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Mouse APOA1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse APOA1 is greater than 95% as determined by SEC-HPLC. |
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