Mouse GIP Protein (GIP-MM101) Summary
Product Description |
Recombinant Mouse GIP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Gln85. |
Product Background |
The potential application of glucose-dependent insulinotropic polypeptide (gastric inhibitory polypeptide, GIP) in the management of obesity and type 2 diabetes has been controversial. Initial interest in the therapeutic use of GIP was dampened by evidence that its insulinotropic activity was reduced in type 2 diabetes and by reports that it increased glucagon secretion and adipose deposition in non-diabetic individuals. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse GIP Protein (GIP-MM101) Specifications/Details
Protein |
GIP |
Synonyms |
GIP; Incretin |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Glu22-Gln85 |
Molecular Weight |
The protein has a predicted MW of 8.7 kDa. Due to glycosylation, the protein migrates to 13-18 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse GIP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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