Name: Mouse Glypican 1/GPC1 Protein (GPC-MM111)
Brand: KactusBio
SKU: GPC-MM111-100UG
Price: 315.0 USD
Availability: InStock

Mouse Glypican 1/GPC1 Protein (GPC-MM111)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Asp24-Ser529

Catalog #GPC-MM111

Size

100UG

500UG

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Product Description	Recombinant Mouse Glypican 1/GPC1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp24-Ser529.
Product Background	CAR-T cells targeting glypican-1 (GPC1)-specific human and murine CAR-T cells generated from our original anti-human/mouse GPC1 antibody showed strong antitumor effects in xenogeneic and syngeneic mouse models, respectively. Importantly, the murine CAR-T cells enhanced endogenous T cell responses against a non-GPC1 tumor antigen through the mechanism of antigen-spreading and showed synergistic antitumor effects with anti-PD-1 antibody without any adverse effects in syngeneic models.
Product Category	Recombinant Protein

Target	Glypican 1/GPC1
Synonyms	Glypican-1; FLJ38078; GPC1
Accession	Q9QZF2
Species	Mouse
Biotinylated	no
Amino Acid Range	Asp24-Ser529
Molecular Weight	The protein has a predicted MW of 56.8 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Bis-Tris PAGE result.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 50mM Tris,150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse Glypican 1/GPC1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse Glypican 1/GPC1 is greater than 95% as determined by SEC-HPLC.

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