Product Description |
Recombinant Mouse&Human Chimeric Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Phe31-Val988 (ITGAV) acidic tail and Gly22-Asn707 (ITGB6) basic tail. |
Product Background |
ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. ITGAV&ITGB6 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. |
Product Category |
Recombinant Protein |
Target |
Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer |
Accession |
|
Species |
Mouse&Human |
Biotinylated |
no |
Amino Acid Range |
Phe31-Val988 (ITGAV) acidic tail & Gly22-Asn707(ITGB6) basic tail |
Molecular Weight |
The protein has a predicted MW of 131.48 kDa (ITGAV) and 79.1 kDa (ITGB6). Due to glycosylation, the protein migrates to 120-160 kDa (ITGAV) and 90-120 kDa (ITGB6) based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse&Human Chimeric ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 26.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Mouse&Human Chimeric ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 26.5ng/ml determined by ELISA. |
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Mouse&Human Chimeric ITGAV&ITGB6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse&Human Chimeric ITGAV&ITGB6 is greater than 95% as determined by SEC-HPLC. |
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