Product Description |
Recombinant Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide. |
Product Background |
HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition. |
Product Category |
Recombinant Protein |
Target |
HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer |
Synonyms |
HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G |
MHC Form |
Tetramer |
Allele |
HLA-G |
Accession |
O02948(HLA-G)&Q6V7J5(B2M)&RIIPRHLQL |
Species |
Rhesus macaque |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide |
Molecular Weight |
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result. |
Activity |
Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 91.9ng/ml determined by ELISA (QC Test). The affinity constant of 1.74 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag with an affinity constant of 1.74 nM as determined in SPR assay (Biacore T200). |
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Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 91.9ng/ml determined by ELISA (QC Test). |
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Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%. |
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The purity of Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer is greater than 95% as determined by SEC-HPLC. |
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