Name: Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein (HLG-RM41CT)
Brand: KactusBio
SKU: HLG-RM41CT-100UG
Price: 825.0 USD
Availability: InStock

Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein (HLG-RM41CT)

Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag with an affinity constant of 1.74 nM as determined in SPR assay (Biacore T200).

Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 91.9ng/ml determined by ELISA (QC Test).

Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.

The purity of Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 91.9ng/ml determined by ELISA (QC Test). The affinity constant of 1.74 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide

Catalog #HLG-RM41CT

Size

100UG

500UG

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Product Description	Recombinant Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide.
Product Background	HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer
Synonyms	HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G
MHC Form	Tetramer
Allele	HLA-G
Accession	O02948(HLA-G)&Q6V7J5(B2M)&RIIPRHLQL
Species	Rhesus macaque
Biotinylated	no
Amino Acid Range	Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide
Molecular Weight	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Activity	Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 91.9ng/ml determined by ELISA (QC Test). The affinity constant of 1.74 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag with an affinity constant of 1.74 nM as determined in SPR assay (Biacore T200).
	Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 91.9ng/ml determined by ELISA (QC Test).
	Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.
	The purity of Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer is greater than 95% as determined by SEC-HPLC.