SARS-COV-2 Spike RBD ( N501Y, K417N, E484K ) Protein (COV-VM1RM)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized SARS-COV-2 Spike RBD (N501Y, K417N, E484K), His Tag at 0.5μg/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.02μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Arg319-Phe541 (N501Y, K417N, E484K)

Catalog: COV-VM1RM

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Product Description Recombinant SARS-COV-2 Spike RBD ( N501Y, K417N, E484K ) Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Arg319-Phe541 (N501Y, K417N, E484K).
Product Category Recombinant Protein
Target Spike RBD ( N501Y, K417N, E484K )
Synonyms S protein RBD; S glycoprotein RBD; Spike protein RBD
Gene/Protein ID QHD43416.1
Species SARS-COV-2
Exact Sequence Arg319-Phe541 (N501Y, K417N, E484K)
Molecular Weight The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
Activity Immobilized SARS-COV-2 Spike RBD (N501Y, K417N, E484K), His Tag at 0.5μg/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.02μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-COV-2 Spike RBD (N501Y, K417N, E484K), His Tag at 0.5μg/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.02μg/ml determined by ELISA.
The purity of SARS-COV-2 Spike RBD ( N501Y, K417N, E484K ) is greater than 95% as determined by SEC-HPLC.
SARS-COV-2 Spike RBD ( N501Y, K417N, E484K ) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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