Name: SARS-COV-2 Spike RBD (N501Y, K417N, E484K) Protein (COV-VM1RM)
Brand: KactusBio
SKU: COV-VM1RM-100UG
Price: 412.5 USD
Availability: InStock

SARS-COV-2 Spike RBD (N501Y, K417N, E484K) Protein (COV-VM1RM)

Immobilized SARS-COV-2 Spike RBD (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.

SARS-COV-2 Spike RBD (N501Y,K417N,E484K) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of SARS-COV-2 Spike RBD (N501Y,K417N,E484K) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized SARS-COV-2 Spike RBD (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Arg319-Phe541(N501Y,K417N,E484K)

Catalog #COV-VM1RM

Size

100UG

500UG

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Product Description	Recombinant SARS-COV-2 Spike RBD (N501Y,K417N,E484K) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(N501Y,K417N,E484K).
Product Background	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Spike RBD (N501Y, K417N, E484K)
Synonyms	S protein RBD; S glycoprotein RBD; Spike protein RBD
Accession	QHD43416.1
Species	SARS-COV-2
Biotinylated	no
Amino Acid Range	Arg319-Phe541(N501Y,K417N,E484K)
Molecular Weight	The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized SARS-COV-2 Spike RBD (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized SARS-COV-2 Spike RBD (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
	SARS-COV-2 Spike RBD (N501Y,K417N,E484K) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of SARS-COV-2 Spike RBD (N501Y,K417N,E484K) is greater than 95% as determined by SEC-HPLC.