SARS-COV-2 Spike RBD (N501Y, K417N, E484K) Protein (COV-VM1RM)
SARS-COV-2 Spike RBD (N501Y, K417N, E484K) Protein (COV-VM1RM)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-COV-2 Spike RBD (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Arg319-Phe541(N501Y,K417N,E484K)
Catalog #COV-VM1RM
Regular price $412.50
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Product Description

Recombinant SARS-COV-2 Spike RBD (N501Y,K417N,E484K) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(N501Y,K417N,E484K).

Product Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

Spike RBD (N501Y, K417N, E484K)

Synonyms

S protein RBD; S glycoprotein RBD; Spike protein RBD

Accession

Species

SARS-COV-2

Biotinylated

no

Amino Acid Range

Arg319-Phe541(N501Y,K417N,E484K)

Molecular Weight

The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.

Activity

Immobilized SARS-COV-2 Spike RBD (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized SARS-COV-2 Spike RBD (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. Immobilized SARS-COV-2 Spike RBD (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
SARS-COV-2 Spike RBD (N501Y,K417N,E484K) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. SARS-COV-2 Spike RBD (N501Y,K417N,E484K) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-COV-2 Spike RBD (N501Y,K417N,E484K) is greater than 95% as determined by SEC-HPLC. The purity of SARS-COV-2 Spike RBD (N501Y,K417N,E484K) is greater than 95% as determined by SEC-HPLC.

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