TelN Protelomerase (TLN-BE001) Summary
Product Description |
TelN Protelomerase is cloned from bacteriophage N15. TelN cuts dsDNA at the recognition site TelRL (56 bp), which consists of a palindromic sequence-TelO in the middle and palindromic sequences R3 and L3 at both ends of 14 bp form. TelN has cutting-ligating enzyme activity and covalently ligates at the cleavage site forming hairpin termini. |
TelN Protelomerase (TLN-BE001) Specifications/Details
Synonyms |
TelN; Phage N15 protelomerase; Protelomerase; TelN enzyme; TelN protein; |
Applications |
In vitro enzymatic synthesis of DNA constructs |
Concentration |
10U/µL |
Unit Definition |
One unit is defined as the amount of enzyme required to cut 0.5 μg completely BsaI linearized pMiniT-TelN control plasmid (313 fmol TelN recognition site) at 30°C for 30 min in a total reaction volume of 20 μL. |
Source |
E.coli strain that carries TelN from phage N15 |
Biotinylated |
no |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice |
Stability And Storage |
-20±5°C for 12 months. Avoid repeated freeze-thaw cycles. |
Validation Images
|
|
TelN Protelomerase cut substrate DNA containing 513 fmol cleaveage site, followed by agarose gel analysis. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
