BsaI, GMP Grade (GMP-BSA-EE101)

  • Quality Statement:
    Special formulation enables BSA-free reaction

    Manufactured to the GMP guidelines

    Antibiotic-free and animal-free production

    Ideal for manufacturing of mRNA therapeutics and vaccines
  • Express System: E.coli

Catalog: GMP-BSA-EE101

$563.00
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Product Description BsaI GMP grade is designed to be used upstream of the in-vitro mRNA synthesis step during mRNA therapeutics and vaccine manufacturing. As one of the type IIS restriction enzymes, it recognizes asymmetric DNA sequences and cleaves outside of their recognition sequence.
The cleavage site of BsaI is GGTCTC(N1/N5), where the GGTCTC acts as the recognition site, and the N1/N5 represents the cleavage site with a 5' overhang. Kacuts provides a BSA-free reaction buffer to ensure the safety of mRNA vaccine production.
Expressed in an E.coli strain that carries the BsaI gene from Bacillus stearothermophilus.
Product Category GMP Enzyme
Application Molecular cloning
Genotyping
SNP
Plasmid linearization
Concentration 20 U/UL
Unit Definition One unit is defined as the amount of BsaI to digest 1UG of λDNA Dcm–-HindIII fragments in 1 hour at 37°C in a 50UL system.
Express System E.coli
Quality Statement
Special formulation enables BSA-free reaction

Manufactured to the GMP guidelines

Antibiotic-free and animal-free production

Ideal for manufacturing of mRNA therapeutics and vaccines
QC Standard Activity: >=20 kU/ml
Purity: >=95%
Residual Endonuclease: Negative
Residual Exonuclease: Negative
Residual Protease: Negative
Endotoxin: <=10 EU/ml
Residual DNase: Negative
Residual RNase: Negative
Residual Host Protein: <=20 ng/MG
Residual Host Cell DNA: <=100 pg/ml
Bioburden: <=1 CFU/10 ml
Form Liquid
Shipping Shipped with blue ice
Stability And Storage -20°C for 12 months. Avoid repeated freeze-thaw cycles.
Fig.2 Add 1μg plasmid and 1μl BsaI (1-10 lane was diluted by 2 times gradient, 1 lane as stock solution) in 30 min at 37℃ in a total reaction volume of 20μl.
Fig.3 Add 1μg plasmid and 1μl BsaI (lane 1-12 was diluted by 2 times gradient, lane 1 as stock solution) in 16 h at 37℃ in a total reaction volume of 20μl.

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