BsaI, GMP Grade (GMP-BSA-EE101)
Catalog: GMP-BSA-EE101
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| Product Description | BsaI GMP grade is designed to be used upstream of the in-vitro mRNA synthesis step during mRNA therapeutics and vaccine manufacturing. As one of the type IIS restriction enzymes, it recognizes asymmetric DNA sequences and cleaves outside of their recognition sequence. The cleavage site of BsaI is GGTCTC(N1/N5), where the GGTCTC acts as the recognition site, and the N1/N5 represents the cleavage site with a 5' overhang. Kacuts provides a BSA-free reaction buffer to ensure the safety of mRNA vaccine production. Expressed in an E.coli strain that carries the BsaI gene from Bacillus stearothermophilus. |
| Product Category | GMP Enzyme |
| Application | Molecular cloning Genotyping SNP Plasmid linearization |
| Concentration | 20 U/UL |
| Unit Definition | One unit is defined as the amount of BsaI to digest 1UG of λDNA Dcm–-HindIII fragments in 1 hour at 37°C in a 50UL system. |
| Express System | E.coli |
| Quality Statement | Special formulation enables BSA-free reaction Manufactured to the GMP guidelines Antibiotic-free and animal-free production Ideal for manufacturing of mRNA therapeutics and vaccines |
| QC Standard | Activity: >=20 kU/ml
Purity: >=95% Residual Endonuclease: Negative Residual Exonuclease: Negative Residual Protease: Negative Endotoxin: <=10 EU/ml Residual DNase: Negative Residual RNase: Negative Residual Host Protein: <=20 ng/MG Residual Host Cell DNA: <=100 pg/ml Bioburden: <=1 CFU/10 ml |
| Form | Liquid |
| Shipping | Shipped with blue ice |
| Stability And Storage | -20°C for 12 months. Avoid repeated freeze-thaw cycles. |
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Fig.2 Add 1μg plasmid and 1μl BsaI (1-10 lane was diluted by 2 times gradient, 1 lane as stock solution) in 30 min at 37℃ in a total reaction volume of 20μl. |
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Fig.3 Add 1μg plasmid and 1μl BsaI (lane 1-12 was diluted by 2 times gradient, lane 1 as stock solution) in 16 h at 37℃ in a total reaction volume of 20μl. |
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