Phi29 DNA Polymerase (PHI-BE101)

Catalog: PHI-BE101


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Product Description Phi29 DNA polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29, with strong strand displacement activity and high processivity (up to more than 70 kbp). The enzyme has inherent 3'-5' exonuclease activity and higher fidelity than most Taq enzymes. It is used for isothermal amplification in vitro.
Synonyms Phi29 enzyme; Phi29 protein; Phi29 polymerase; ϕ29 dna polymerase; ϕ29 polymerase; ϕ29 enzyme
Application Multiple strand displacement amplification (MDA)
Whole genome amplification (WGA)
Rolling circle amplification
Concentration 10U/µL
Unit Definition One unit is defined as the amount of enzyme required to incorporate 0.5 pmol of dNTPs into acid-insoluble substances within 10 minutes in a total reaction volume of 50µL at 30°C.
Source E. coli strain carrying the phi29 DNA polymerase gene cloned from Bacillus subtilis phage phi29
Form Liquid
Shipping Shipped on dry ice.
Stability And Storage -20±5°C for 24 months. Avoid repeated freeze-thaw cycles.
Phi29 DNA Polymerase on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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