Product Description |
Phi29 DNA polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29, with strong strand displacement activity and high processivity (up to more than 70 kbp). The enzyme has inherent 3'-5' exonuclease activity and higher fidelity than most Taq enzymes. It is used for isothermal amplification in vitro. |
Synonyms |
Phi29 enzyme; Phi29 protein; Phi29 polymerase; ϕ29 dna polymerase; ϕ29 polymerase; ϕ29 enzyme |
Applications |
Multiple strand displacement amplification (MDA) Whole genome amplification (WGA) Rolling circle amplification |
Concentration |
10U/µL |
Unit Definition |
One unit is defined as the amount of enzyme required to incorporate 0.5 pmol of dNTPs into acid-insoluble substances within 10 minutes in a total reaction volume of 50µL at 30°C. |
Source |
E. coli strain carrying the phi29 DNA polymerase gene cloned from Bacillus subtilis phage phi29 |
Biotinylated |
no |
Form |
Liquid |
Shipping |
Shipped on dry ice. |
Stability And Storage |
-20±5°C for 24 months. Avoid repeated freeze-thaw cycles. |
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|
Rolling cycle amplification of pUC57 plasmid with phi29 DNA Polymerase, product was digested with BspQI and then analyzed with Agarose gel. |
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