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Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E Protein (VZV-VM10E) Summary
Product Description |
Recombinant Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser31-Tyr538. |
Product Background |
VZV glycoprotein E (gE) is most abundantly expressed on the surface of infected cells, and is an essential component for virus replication and cell-to-cell transmission. It is also the main target of virus-specific antibodies and T cell responses that is often selected as vaccine candidate antigen. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E Protein (VZV-VM10E) Specifications/Details
Protein |
Envelope glycoprotein E |
Synonyms |
Envelope glycoprotein E; gE; ORF68; |
Accession |
|
Species |
Varicella-zoster virus (strain Oka vaccine) |
Biotinylated |
no |
Amino Acid Range |
Ser31-Tyr538 |
Molecular Weight |
The protein has a predicted MW of 58.39 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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