Product Description |
Recombinant Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E Protein is expressed from HEK293 with His tag at the C-Terminus.; It contains Ser31-Tyr538. |
Product Background |
VZV glycoprotein E (gE) is most abundantly expressed on the surface of infected cells, and is an essential component for virus replication and cell-to-cell transmission. It is also the main target of virus-specific antibodies and T cell responses that is often selected as vaccine candidate antigen. |
Product Category |
Recombinant Protein |
Target |
Envelope glycoprotein E |
Accession |
|
Species |
Varicella-zoster virus (strain Oka vaccine) |
Biotinylated |
no |
Amino Acid Range |
Ser31-Tyr538 |
Molecular Weight |
The protein has a predicted MW of 58.39 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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