Product Description |
DNase I is an endonuclease that digests single- and double-stranded DNA to oligonucleotide containing a 5' phosphate and 3'-hydroxylated ends. It is an essential enzyme for the efficient digestion of DNA during RNA purification and is suitable for molecular diagnostics and in vitro mRNA synthesis applications. This product has been submitted to the FDA Drug Master Files and is assigned DMF #038032. |
Synonyms |
DNase1; DNase 1; DNaseI; Deoxyribonuclease I; DeoxyribonucleaseI; Deoxyribonuclease 1; Deoxyribonuclease1; |
Applications |
In Vitro Transcription (IVT) Removal of DNA from RNA samples DNase I footprinting |
Concentration |
4U/µL |
Unit Definition |
One unit corresponds to the amount of enzyme required to produce a change in absorbance at 260 nm of 1.0 in 10 minutes. |
Source |
Expressed in an E.coli strain with DNase I gene isolated from bovine pancreas. |
Biotinylated |
no |
Molecular Weight |
39kD |
Quality Standards |
Activity (Degradation plasmid method): ≥ 4 kU/mL
Purity (SEC-HPLC): ≥ 95% Residual RNase: Negative Residual Protease: Negative Endotoxin: ≤ 1.2 EU/mL Residual Host Cell DNA: ≤ 100 pg/mg Residual Host protein: ≤ 20 ng/mg Residual Heavy Metal: ≤ 10 ppm Residual Nickel Salt: ≤ 10 ppm Bioburden: ≤ 1 CFU/10 mL |
Form |
Liquid |
Shipping |
Shipping with dry ice |
Stability And Storage |
-20C±5°C for 24 months. |
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Figure 1. 1µg DNA was added to various activity units of DNase I in a total reaction volume of 10µL. Compared with competitors 1 and 2, DNase I of KACTUS has a stronger digestive effect. |
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Figure 2. Different concentrations of ions were added to the transcriptional reaction. Results show DNase I is inhibited by Na+ and K+. |
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