Product Description |
Recombinant Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus). |
Product Background |
ActRIIB (activin receptor type-2B) is an activin receptor subtype constitutively expressed in the whole body, playing a role in cellular proliferation, differentiation, and metabolism. For its various physiological activities, ActRIIB interacts with activin and multiple other ligands including myostatin (MSTN), growth differentiation factor 11 (GDF11), and bone morphogenetic protein 9 (BMP9). |
Product Category |
Recombinant Protein |
Target |
Activin RIIB/ACVR2B |
Accession |
|
Species |
Human/Cynomolgus |
Biotinylated |
yes |
Amino Acid Range |
Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus) |
Molecular Weight |
The protein has a predicted MW of 16.56 kDa. Due to glycosylation, the protein migrates to 33-43 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Activin A, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 53.8ng/ml determined by ELISA (QC Test). The affinity constant of 6.44 pM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Human/Cynomolgus Activin RIIB, His Tag immobilized on CM5 Chip can bind Anti-Activin RIIB Antibody, hFc Tag with an affinity constant of 6.44 pM as determined in SPR assay (Biacore T200). |
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Biotinylated Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Activin A, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200). |
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Immobilized Human Activin A, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 53.8ng/ml determined by ELISA (QC Test). |
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Immobilized Biotinylated Human/Cynomolgus Activin RIIB, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Activin RIIB Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. |
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Biotinylated Human/Cynomolgus Activin RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human/Cynomolgus Activin RIIB is greater than 95% as determined by SEC-HPLC. |
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