Cynomolgus B7-1/CD80 Protein (B71-CM280)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus B7-1, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val35-Asn242
Catalog #B71-CM280
Regular price $225.00
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Product Description
Recombinant Cynomolgus B7-1/CD80 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Val35-Asn242.
Product Background
Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
Product Category
Recombinant Protein
Target
B7-1/CD80
Synonyms
CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Val35-Asn242
Molecular Weight
The protein has a predicted MW of 49.84 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus B7-1, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-hFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus B7-1, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA.
Cynomolgus B7-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus B7-1 is greater than 95% as determined by SEC-HPLC.

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