Product Description |
Recombinant Cynomolgus B7-1/CD80 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Val35-Asn242. |
Product Background |
Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4. |
Product Category |
Recombinant Protein |
Target |
B7-1/CD80 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Val35-Asn242 |
Molecular Weight |
The protein has a predicted MW of 49.84 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus B7-1, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Cynomolgus CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus B7-1, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. |
|
|
Cynomolgus B7-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Cynomolgus B7-1 is greater than 95% as determined by SEC-HPLC. |
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