Human LY6E Protein (LYE-HM306)
Human LY6E Protein (LYE-HM306)
Human LY6E Protein (LYE-HM306)
  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LY6E, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY6E Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu21-Ser101
Catalog #LYE-HM306
Regular price $315.00
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Product Description
Recombinant Human LY6E Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminus.It contains Leu21-Ser101.
Product Background
Lymphocyte antigen 6 complex, locus E (LY6E), also designated as stem cell antigen 2 (SCA2) and thymic shared antigen-1 (TSA-1), is a member of the lymphostromal cell membrane Ly6 superfamily. All Ly6 family members are low-molecular weight (10-12 kDa) proteins that display limited nucleotide and amino acid sequence homologies with one another, but commonly have a conserved cysteine-rich cell surface domain and are anchored to the cell surface via a glycosilphosphatidylinositol (GPI) moiety.
Product Category
Recombinant Protein
Target
LY6E
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Leu21-Ser101
Molecular Weight
The protein has a predicted MW of 34.15 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human LY6E, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY6E Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-mFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human LY6E, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY6E Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA.
Human LY6E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LY6E is greater than 95% as determined by SEC-HPLC.

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