Human IL-17A&F Protein, Ultra Low Endotoxin (IAF-HM117-UL)

Express System: HEK293
Product Tag: N-Strep-II, N-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 62.6ng/ml determined by ELISA.
Amino Acid Range: Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F)

Catalog #IAF-HM117-UL

Price

$380.00

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Human IL-17A&F Protein, Ultra Low Endotoxin (IAF-HM117-UL) Summary

Product Description	Recombinant Human IL-17A&F Protein is expressed as a heterodimer of IL-17A and IL-17F by co-expression in HEK293. Subunit IL-17A is fused with a Strep II tag at the N-terminus and subunit IL-17F is fused with a His tag at the N-terminus.It contains Gly24-Ala155 (IL-17A) and Arg31-Gln163 (IL-17F).
Product Background	Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Protein	IL-17A&F
Synonyms	IL-17A,Interleukin-17A,CTLA-8,IL-17,IL-17F,IL24,ML-1,Interleukin-17F
Accession	Q16552-1(IL-17A)&Q96PD4(IL-17F)
Species	Human
Biotinylated	no
Amino Acid Range	Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F)
Molecular Weight	The protein has a predicted MW of 16.16 kDa (IL-17A) and 16 kDa (IL-17F). Due to glycosylation, the protein migrates to 17-23 kDa and 25-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 62.6ng/ml determined by ELISA.
Product Tag	N-Strep-II, N-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 62.6ng/ml determined by ELISA.
	Immobilized Human IL-17A&F, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag (Cat. IL1-HM2RC) with the EC50 of 85.2ng/ml determined by ELISA.
	Immobilized Human IL-17A&F, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 1.3ng/ml determined by ELISA.
	Human IL-17A&F on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human IL-17A&F is greater than 95% and the molecular weight of this protein is around 37-50 kDa as determined by SEC-MALS.
	The purity of Human IL-17A&F is greater than 95% as determined by SEC-HPLC.

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