Restriction Endonucleases
KACTUS offers a selection of GMP-Grade and GMP-Ready Restriction Endonucleases for plasmid linearization prior to in vitro transcription. Our restriction enzymes are expressed in E. coli and have high purity and batch consistency.
GMP-Grade: Manufactured according to cGMP guidelines.
GMP-Ready: Industrial-grade manufacturing transferrable to GMP-level manufacturing.
| Enzyme | Recognition Site | Grade | Catalog # |
|---|---|---|---|
| BsaI | 5’...GGTCTC(N)₁↓...3’ 3’...CCAGAG(N)₅↑...5’ | GMP-Grade DMF #037503 | GMP-BSA-EE101 |
| BspQI | 5’...GCTCTTC(N)₁↓...3’ 3’...CGAGAAG(N)₄↑...5’ | GMP-Ready | BSP-BE101 |
| I-SceI | 5’...TAGGGATAA↓CAGGGTAAT...3’ 3’...ATCCC↑TATTGTCCCATTA...5’ | GMP-Ready | ISC-YE101 |
| KpnI | 5’...GGTAC↓C...3’ 3’...C↑CATGG...5’ | GMP-Ready | KPN-KE101 |
| NruI | 5’…TCG↓CGA…3’ 3’…AGC↑GCT…5’ | GMP-Ready | NRU-RE101 |
| PvuII | 5’ …CAG↓CTG… 3’ 3’ …GTC↑GAC… 5’ | GMP-Ready | PVU-PE101 |
| SalI | 5’...G↓TCGAC...3’ 3’...CAGCT↑G...5’ | GMP-Ready | SAL-SE101 |
| SapI | 5’...GCTCTTC(N)₁↓...3’ 3’...CGAGAAG(N)₄↑...5’ | GMP-Ready | SAP-SE101 |
| XbaI | 5’ ...T↓CTAGA... 3’ 3’ ...AGATC↑T... 5’ | GMP-Ready | XBA-EE101 |