• Express System: HEK293
  • Product Tag: N-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA.
  • Amino Acid Range: Ala108-Gly220
Catalog #ARN-HM201
Price
Regular price $33000
Regular price Sale price $33000
/
Size
Avantor VWR Fisher Scientific

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Human ARTN Protein (ARN-HM201) Summary

Product Description

 Recombinant Human ARTN Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Ala108-Gly220.

Product Background

 Artemin (ARTN) is a member of glial cell line-derived neurotrophic factor (GDNF) family of ligands, and its signaling is mediated via a multi-component receptor complex including the glycosylphosphatidylinositol-anchored GDNF family receptors a (GFRa1, GFRa3) and RET receptor tyrosine kinase. The major mechanism of ARTN action is via binding to a non-signaling co-receptor. The major function of ARTN is to drive the molecule to induce migration and axonal projection from sympathetic neurons.

Human ARTN Protein (ARN-HM201) Specifications/Details

Protein
 ARTN
Synonyms
 Artemin; Enovin; Neublastin; ARTNE; EVN; ARTN; NBN
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala108-Gly220
Molecular Weight
 The protein has a predicted MW of 39.3 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA.
Product Tag
 N-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in 4mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA. Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA.
Human ARTN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human ARTN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human GFRA3, His Tag on Anti-His-Biosensor can bind Human ARTN, hFc Tag with an affinity constant of 5.95 nM as determined in BLI assay. Loaded Human GFRA3, His Tag on Anti-His-Biosensor can bind Human ARTN, hFc Tag with an affinity constant of 5.95 nM as determined in BLI assay.
The purity of Human ARTN is greater than 90% as determined by SEC-HPLC. The purity of Human ARTN is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...