• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. The specific activity is >3.5 pmol/min/µg.
  • Amino Acid Range: Thr22-Thr457
Catalog #BAE-HM101
Price
Regular price $239.80
Regular price Sale price $239.80
/
Size
Avantor VWR Fisher Scientific

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Human BACE-1 Protein (BAE-HM101) Summary

Product Description

 Recombinant Human BACE-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr22-Thr457.

Product Background

 The beta-site amyloid precursor protein cleaving enzyme-1 (BACE-1) initiates the generation of amyloid-β (Aβ), and the amyloid cascade leading to amyloid plaque deposition, neurodegeneration, and dementia in Alzheimer's disease (AD). Clinical failures of anti-Aβ therapies in dementia stages suggest that treatment has to start in the early, asymptomatic disease states.

Human BACE-1 Protein (BAE-HM101) Specifications/Details

Protein
 BACE-1
Synonyms
 Beta-secretase 1; ASP2; Asp 2; BACE1; BACE; FLJ90568; HSPC104; KIAA1149; Memapsin-2; BACE-1; EC 3.4.23; EC 3.4.23.46;β-secretase 1;EC3.4.23; EC3.4.23.46;b-secretase 1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Thr22-Thr457
Molecular Weight
 The protein has a predicted MW of 49.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
 Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. The specific activity is >3.5 pmol/min/µg.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human BACE-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human BACE-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human BACE-1 is greater than 95% as determined by SEC-HPLC. The purity of Human BACE-1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...