• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus NCAM-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Leu20-Gly718
Catalog #CAM-CM156
Price
Regular price $346.50
Regular price Sale price $346.50
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Size
Avantor VWR Fisher Scientific

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Cynomolgus NCAM-1/CD56 Protein (CAM-CM156) Summary

Product Description

 Recombinant Cynomolgus NCAM-1/CD56 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu20-Gly718.

Product Background

 Neural Cell Adhesion Molecule 1 (NCAM-1), a multifunctional member of the immunoglobulin superfamily, is expressed on the surface of neurons, glia, skeletal muscle, and natural killer cells. NCAM-1 has been implicated as having a role in cell-cell adhesion, involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.

Cynomolgus NCAM-1/CD56 Protein (CAM-CM156) Specifications/Details

Protein
 NCAM-1/CD56
Synonyms
 N-CAM-1; NCAM-1; NCAM1; CD56; NCAM; MSK39;N-CAM1
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Leu20-Gly718
Molecular Weight
 The protein has a predicted MW of 78.46 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus NCAM-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus NCAM-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). Immobilized Cynomolgus NCAM-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).
Cynomolgus NCAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus NCAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus NCAM-1 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus NCAM-1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...