• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Fc gamma RIIIA (F176) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.92uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gly17-Gln208(F176)
Catalog #CDA-HM416
Price
Regular price $264.00
Regular price Sale price $264.00
/
Size
Avantor VWR Fisher Scientific

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Non-biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein (CDA-HM416) Summary

Product Description

 Recombinant Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus..It contains Gly17-Gln208(F176).

Product Background

 Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Product Category

 Recombinant Protein / Antibody discovery / Fc receptors

Non-biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein (CDA-HM416) Specifications/Details

Protein
 Fc gamma RIIIA/CD16a
Synonyms
 Fc gamma RIIIA; Fc gamma RIIIA (V176); FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20; FcγRIIIA; Fc γRIIIA; Fcγ RIIIA; Fc γ RIIIA
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly17-Gln208(F176)
Molecular Weight
 The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Fc gamma RIIIA (F176) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.92uM as determined in SPR assay (Biacore T200).
Product Tag
 C-His-Avi

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via anti-His antibody can bind Rituximab, hFc Tag with an affinity constant of 0.92uM as determined in SPR assay (Biacore T200). Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via anti-His antibody can bind Rituximab, hFc Tag with an affinity constant of 0.92uM as determined in SPR assay (Biacore T200).
Immobilized Human Fc gamma RIIIA (F176) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA (QC Test). Immobilized Human Fc gamma RIIIA (F176) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA (QC Test).
Human Fc gamma RIIIA (F176) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human Fc gamma RIIIA (F176) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC. The purity of Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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