• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Cynomolgus CRTAM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CRTAM Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA (QC Test)
  • Amino Acid Range: Ser18-Gly287
Catalog #CRM-CM101
Price
Regular price $26400
Regular price Sale price $26400
/
Size
Avantor VWR Fisher Scientific

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Cynomolgus CRTAM Protein (CRM-CM101) Summary

Product Description

 Recombinant Cynomolgus CRTAM Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser18-Gly287.

Product Background

 Class-I Restricted T Cell-Associated Molecule (CRTAM) is a protein that is expressed after T cell activation. The interaction of CRTAM with its ligand, nectin-like 2 (Necl2), is required for the efficient production of IL-17, IL-22, and IFNγ by murine CD4 T cells, and it plays a role in optimal CD8 T and NK cell cytotoxicity. CRTAM promotes the pro-inflammatory cytokine profile; therefore, it may take part in the immunopathology of autoimmune diseases such as diabetes type 1 or colitis.

Cynomolgus CRTAM Protein (CRM-CM101) Specifications/Details

Protein
 CRTAM
Synonyms
 CD355 antigen; CD355; CRTAM
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Ser18-Gly287
Molecular Weight
 The protein has a predicted MW of 30.80 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus CRTAM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CRTAM Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA (QC Test)
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus CRTAM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CRTAM Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA (QC Test) Immobilized Cynomolgus CRTAM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CRTAM Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA (QC Test)
Cynomolgus CRTAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus CRTAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
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