• Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Cynomolgus Galectin 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.
  • Amino Acid Range: Ala2-Ile248
Catalog #GLT-CM103
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Cynomolgus Galectin 3 Protein (GLT-CM103) Summary

Product Description

 Recombinant Cynomolgus Galectin 3 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ala2-Ile248.

Product Background

 Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain.Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity).

Cynomolgus Galectin 3 Protein (GLT-CM103) Specifications/Details

Protein
 Galectin 3
Synonyms
 AGE-R3; CBP35; GAL3; Gal-3; galactin-3; GALBPCBP35; Galectin3; GALIG; L29; L31; Lectin L-29; LGALS2; LGALS3; Mac-2; MAC2GAL3;Lectin L29; Mac2
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Ala2-Ile248
Molecular Weight
 The protein has a predicted MW of 27.05 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus Galectin 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.
Product Tag
 N-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 50mM Hepes, 150mM NaCl, 200mM L-arginine, 2mM Tcep (pH 6.5). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus Galectin 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. Immobilized Cynomolgus Galectin 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.
Cynomolgus Galectin 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus Galectin 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
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