• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Her3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.33ug/ml determined by ELISA (QC Test).
  • Amino Acid Range: Ser20-Thr643
Catalog #HER-HM203
Price
Regular price $19800
Regular price Sale price $19800
/
Size
Avantor VWR Fisher Scientific

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Human Her3/ErbB3 Protein (HER-HM203) Summary

Product Description

 Recombinant Human Her3/ErbB3 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ser20-Thr643.

Product Background

 Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).

Human Her3/ErbB3 Protein (HER-HM203) Specifications/Details

Protein
 Her3/ErbB3
Synonyms
 ErbB3; ErbB-3; HER3; HER3c-erbB-3; LCCS2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; ERBB3;HER3c-erbB3; MDA-BF1; p180ErbB3; p45sErbB3; p85sErbB3
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser20-Thr643
Molecular Weight
 The protein has a predicted MW of 95.4 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Her3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.33ug/ml determined by ELISA (QC Test).
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Her3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.33ug/ml determined by ELISA (QC Test). Immobilized Human Her3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.33ug/ml determined by ELISA (QC Test).
Immobilized Human Her3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Her3 Antibody, hFc Tag with the EC50 of 10.7ng/ml determined by ELISA. Immobilized Human Her3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Her3 Antibody, hFc Tag with the EC50 of 10.7ng/ml determined by ELISA.
Human Her3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human Her3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
Loaded Human NRG1 beta 1, His Tag (Cat. NRG-HM1B1) on Anti-His-Biosensor can bind Human Her3, hFc Tag with an affinity constant of 2.61 nM as determined in BLI assay. Loaded Human NRG1 beta 1, His Tag (Cat. NRG-HM1B1) on Anti-His-Biosensor can bind Human Her3, hFc Tag with an affinity constant of 2.61 nM as determined in BLI assay.
The purity of-Human Her3 is greater than 95% as determined by SEC-HPLC. The purity of-Human Her3 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...