• Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: > 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LIGHT Trimer, His tag at 5ug/ml (100 ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: His90-Val240
Catalog #LGT-HM132
Price
Regular price $297.00
Regular price Sale price $297.00
/
Size
Avantor VWR Fisher Scientific

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Human LIGHT/TNFSF14 Trimer Protein (LGT-HM132) Summary

Product Description

 Recombinant Human LIGHT/TNFSF14 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.; It contains His90-Val240 trimer design.

Product Background

 LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.

Human LIGHT/TNFSF14 Trimer Protein (LGT-HM132) Specifications/Details

Protein
 LIGHT/TNFSF14
Synonyms
 CD258; TNFSF14; HVEM-L; LIGHT; LTg;-TR2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 His90-Val240
Molecular Weight
 The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-140 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Activity
 Immobilized Human LIGHT Trimer, His tag at 5ug/ml (100 ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test).
Product Tag
 N-His-Flag
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human LIGHT Trimer, His tag at 5ug/ml (100 ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test). Immobilized Human LIGHT Trimer, His tag at 5ug/ml (100 ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test).
Human LIGHT Trimer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 90%. Human LIGHT Trimer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 90%.
The purity of Human LIGHT Trimer is greater than 95% as determined by SEC-HPLC. The purity of Human LIGHT Trimer is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...