• Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse NKG2D, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 71.3ng/ml determined by ELISA.
  • Amino Acid Range: Phe90-Val232
Catalog #NKG-MM12D
Price
Regular price $26400
Regular price Sale price $26400
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Mouse NKG2D/CD314 Protein (NKG-MM12D) Summary

Product Description

 Recombinant Mouse NKG2D/CD314 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Phe90-Val232.

Product Background

 NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.

Mouse NKG2D/CD314 Protein (NKG-MM12D) Specifications/Details

Protein
 NKG2D/CD314
Synonyms
 CD314; D12S2489E; KLR; NKG2-D; NKG2D
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Phe90-Val232
Molecular Weight
 The protein has a predicted MW of 17.5 kDa. Due to glycosylation, the protein migrates to 24-35kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse NKG2D, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 71.3ng/ml determined by ELISA.
Product Tag
 N-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 20mM Tris, 250mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Mouse NKG2D, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 71.3ng/ml determined by ELISA. Immobilized Mouse NKG2D, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 71.3ng/ml determined by ELISA.
Mouse NKG2D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse NKG2D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse NKG2D is greater than 95% as determined by SEC-HPLC. The purity of Mouse NKG2D is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...