• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.
  • Amino Acid Range: Met22-Arg734
Catalog #SEM-HM44D
Price
Regular price $33000
Regular price Sale price $33000
/
Size
Avantor VWR Fisher Scientific

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Non-biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein (SEM-HM44D) Summary

Product Description

 Recombinant Human Semaphorin 4D/SEMA4D/CD100 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met22-Arg734.

Product Background

 Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression.

Non-biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein (SEM-HM44D) Specifications/Details

Protein
 Semaphorin 4D/SEMA4D/CD100
Synonyms
 Semaphorin-4D; CD100; SEMA4D; C9orf164; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met22-Arg734
Molecular Weight
 The protein has a predicted MW of 82.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. Immobilized Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.
Human Semaphorin 4D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Semaphorin 4D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Semaphorin 4D is greater than 95% as determined by SEC-HPLC. The purity of Human Semaphorin 4D is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...