• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Met17-Ala363
Catalog #SIG-HM408B
Price
Regular price $577.50
Regular price Sale price $577.50
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human Siglec-8 Protein (SIG-HM408B) Summary

Product Description

 Recombinant Biotinylated Human Siglec-8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met17-Ala363.

Product Background

 Siglec-8, also known as SAF, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains.Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human Siglec-8 Protein (SIG-HM408B) Specifications/Details

Protein
 Siglec-8
Synonyms
 CDw329; MGC59785; SAF2; SAF2SAF-2; Siglec-8; SIGLEC8L; SIGLEC8;SAF2SAF2
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Met17-Ala363
Molecular Weight
 The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His-Avi

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human Siglec-8 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Biotinylated Human Siglec-8 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
Other Available Proteins:
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