• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD48, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human 2B4, hFc Tag (Cat. 2B4-HM201) with the EC50 of 90.1ng/ml determined by ELISA.
  • Amino Acid Range: Gln27-Ser220
Catalog #SLA-HM148
Price
Regular price $28050
Regular price Sale price $28050
/
Size
Avantor VWR Fisher Scientific

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Human CD48/SLAMF2 Protein (SLA-HM148) Summary

Product Description

 Recombinant Human CD48/SLAMF2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln27-Ser220.

Product Background

 CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules.CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors.

Human CD48/SLAMF2 Protein (SLA-HM148) Specifications/Details

Protein
 CD48/SLAMF2
Synonyms
 CD48 molecule; CD48; SLAMF2; BCM1; BLAST; BLAST1; BLAST1TCT.1;- mCD48; MEM-102; TCT.1;MEM102
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln27-Ser220
Molecular Weight
 The protein has a predicted MW of 23.4 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD48, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human 2B4, hFc Tag (Cat. 2B4-HM201) with the EC50 of 90.1ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CD48, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human 2B4, hFc Tag (Cat. 2B4-HM201) with the EC50 of 90.1ng/ml determined by ELISA. Immobilized Human CD48, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human 2B4, hFc Tag (Cat. 2B4-HM201) with the EC50 of 90.1ng/ml determined by ELISA.
Human CD48 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD48 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human CD48, His Tag on Anti-His-Biosensor can bind Human 2B4, hFc Tag with an affinity constant of 5.79 nM as determined in BLI assay. Loaded Human CD48, His Tag on Anti-His-Biosensor can bind Human 2B4, hFc Tag with an affinity constant of 5.79 nM as determined in BLI assay.
The purity of Human CD48 is greater than 95% as determined by SEC-HPLC. The purity of Human CD48 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...