• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human TIGIT, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD155, hFc Tag (Cat. CD1-HM255) with the EC50 of 2.9ng/ml determined by ELISA.
  • Amino Acid Range: Met22-Pro141
Catalog #TIG-HM410B
Price
Regular price $577.50
Regular price Sale price $577.50
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Biotinylated Human TIGIT Protein (TIG-HM410B) Summary

Product Description

 Recombinant Biotinylated Human TIGIT Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met22-Pro141.

Product Background

 TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.TIGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.

Biotinylated Human TIGIT Protein (TIG-HM410B) Specifications/Details

Protein
 TIGIT
Synonyms
 TIGIT; VSIG9; VSTM3; WUCAM
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Met22-Pro141
Molecular Weight
 The protein has a predicted MW of 16.1 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human TIGIT, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD155, hFc Tag (Cat. CD1-HM255) with the EC50 of 2.9ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human TIGIT, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD155, hFc Tag (Cat. CD1-HM255) with the EC50 of 2.9ng/ml determined by ELISA. Immobilized Biotinylated Human TIGIT, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD155, hFc Tag (Cat. CD1-HM255) with the EC50 of 2.9ng/ml determined by ELISA.
Biotinylated Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human TIGIT is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human TIGIT is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...