• Express System: HEK293
  • Product Tag: N-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TNFRSF12A, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Glu28-Per80
Catalog #TNF-HM20A
Price
Regular price $23100
Regular price Sale price $23100
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Size
Avantor VWR Fisher Scientific

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Human TNFRSF12A/TWEAKR Protein (TNF-HM20A) Summary

Product Description

 Recombinant Human TNFRSF12A/TWEAKR Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Glu28-Per80.

Product Background

 Tumor necrosis factor receptor superfamily member 12a (Tnfrsf12a) is a receptor for tumor necrosis factor superfamily member 12 (Tnfsf12). Tnfrsf12a is highly inducible and plays a key role in the development of cardiac hypertrophy and heart failure.

Human TNFRSF12A/TWEAKR Protein (TNF-HM20A) Specifications/Details

Protein
 TNFRSF12A/TWEAKR
Synonyms
 FGF-inducible 14; Fgfrp2; TNFRSF12A; FN14; CD266; TWEAKR
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu28-Per80
Molecular Weight
 The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human TNFRSF12A, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Product Tag
 N-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNFRSF12A, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA (QC Test). Immobilized Human TNFRSF12A, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA (QC Test).
Human TNFRSF12A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human TNFRSF12A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TNFRSF12A is greater than 95% as determined by SEC-HPLC. The purity of Human TNFRSF12A is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...