• Express System: HEK293
  • Product Tag: N-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TSPAN8, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TSPAN8 Antibody, hFc Avi Tag with the EC50 of 35.7ng/ml determined by ELISA.
  • Amino Acid Range: Lys110-Asn205
Catalog #TSP-HM2N8
Price
Regular price $29700
Regular price Sale price $29700
/
Size
Avantor VWR Fisher Scientific

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Human TSPAN8 Protein (TSP-HM2N8) Summary

Product Description

 Recombinant Human TSPAN8 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Lys110-Asn205.

Product Background

 Tetraspanin 8 (TSPAN8) is a member of the tetraspanin superfamily that forms TSPAN8-mediated protein complexes by interacting with themselves and other various cellular signaling molecules.In physiological conditions, TSPAN8 plays a vital role in the regulation of biological functions, including leukocyte trafficking, angiogenesis and wound repair.

Human TSPAN8 Protein (TSP-HM2N8) Specifications/Details

Protein
 TSPAN8
Synonyms
 Tetraspanin-8; Tetraspanin 8; Tspan-8; Tspan 8; TM4SF3; CO-029; TM4SF3; CO 029;;Tspan8; CO029
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Lys110-Asn205
Molecular Weight
 The protein has a predicted MW of 37.30 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human TSPAN8, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TSPAN8 Antibody, hFc Avi Tag with the EC50 of 35.7ng/ml determined by ELISA.
Product Tag
 N-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human TSPAN8, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TSPAN8 Antibody, hFc Avi Tag with the EC50 of 35.7ng/ml determined by ELISA. Immobilized Human TSPAN8, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TSPAN8 Antibody, hFc Avi Tag with the EC50 of 35.7ng/ml determined by ELISA.
Human TSPAN8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human TSPAN8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TSPAN8 is greater than 95% as determined by SEC-HPLC. The purity of Human TSPAN8 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...