Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) (TSP-CM101B)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Cynomolgus TSLP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Tyr29-Gln159

Catalog: TSP-CM101B

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Product Description Recombinant Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr29-Gln159.

Product Background

 Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Product Category

 Recombinant Protein

Target

 TSLP
Synonyms thymic stromal lymphopoietin; TSLP

Gene/Protein ID

 A0A7N9CAT7

Species

 Cynomolgus

Exact Sequence

 Tyr29-Gln159
Molecular Weight The protein has a predicted MW of 16.3 kDa. Due to glycosylation, the protein migrates to 10-13 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Biotinylated Cynomolgus TSLP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Cynomolgus TSLP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA (QC Test).
Immobilized Cynomolgus TSLPR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus TSLP, His Tag with the EC50 of 0.12ug/ml determined by ELISA.
The purity of Biotinylated Cynomolgus TSLP is greater than 90% as determined by SEC-HPLC.
Biotinylated Cynomolgus TSLP on Tris-Bis PAGE under reduced conditions. The purity is greater than 90%.

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