Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) (TSP-CM101B)
Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) (TSP-CM101B)
Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) (TSP-CM101B)
Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) (TSP-CM101B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Biotinylated Cynomolgus TSLP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Tyr29-Gln159
Catalog #TSP-CM101B
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Product Description
 Recombinant Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr29-Gln159.
Product Background
 Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
Product Category
 Recombinant Protein
Target
 TSLP
Synonyms
 thymic stromal lymphopoietin; TSLP
Accession
Species
 Cynomolgus
Biotinylated
 yes
Amino Acid Range
 Tyr29-Gln159
Molecular Weight
 The protein has a predicted MW of 16.3 kDa. Due to furin cleavage site, the protein migrates to 10-13 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Cynomolgus TSLP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Cynomolgus TSLP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA (QC Test).
Immobilized Cynomolgus TSLPR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus TSLP, His Tag with the EC50 of 0.12ug/ml determined by ELISA.
Biotinylated Cynomolgus TSLP on Bis-Tris PAGE under reduced conditions. The purity is greater than 90%.
The purity of Biotinylated Cynomolgus TSLP is greater than 90% as determined by SEC-HPLC.
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