Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) (TSP-CM101B)
Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) (TSP-CM101B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Biotinylated Cynomolgus TSLP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Tyr29-Gln159
Catalog #TSP-CM101B
Regular price $577.50
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Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) (TSP-CM101B) Summary

Product Description

Recombinant Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr29-Gln159.

Product Background

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) (TSP-CM101B) Specifications/Details

Protein
TSLP
Synonyms
thymic stromal lymphopoietin; TSLP
Accession
Species
Cynomolgus
Biotinylated
yes
Amino Acid Range
Tyr29-Gln159
Molecular Weight
The protein has a predicted MW of 16.3 kDa. Due to furin cleavage site, the protein migrates to 10-13 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Cynomolgus TSLP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Cynomolgus TSLP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Cynomolgus TSLP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA (QC Test).
Immobilized Cynomolgus TSLPR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus TSLP, His Tag with the EC50 of 0.12ug/ml determined by ELISA. Immobilized Cynomolgus TSLPR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus TSLP, His Tag with the EC50 of 0.12ug/ml determined by ELISA.
Biotinylated Cynomolgus TSLP on Bis-Tris PAGE under reduced conditions. The purity is greater than 90%. Biotinylated Cynomolgus TSLP on Bis-Tris PAGE under reduced conditions. The purity is greater than 90%.
Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus TSLP, His Tag with an affinity constant of 13.40 nM as determined in BLI assay (Gator® Prime). Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus TSLP, His Tag with an affinity constant of 13.40 nM as determined in BLI assay (Gator® Prime).
Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus TSLP, His Tag with an affinity constant of 1.12 nM as determined in BLI assay (Gator® Prime). Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus TSLP, His Tag with an affinity constant of 1.12 nM as determined in BLI assay (Gator® Prime).
The purity of Biotinylated Cynomolgus TSLP is greater than 90% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus TSLP is greater than 90% as determined by SEC-HPLC.
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