Biotinylated Human DLL4 Protein (DLL-HM404B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-DLL4 Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human DLL4, His Tag with the EC50 of 0.33μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser27-Pro524

Catalog: DLL-HM404B

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Product Description Recombinant Biotinylated Human DLL4 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Ser27-Pro524.
Product Category Recombinant Protein
Target DLL4
Synonyms DLL4; MGC126344; notch ligand DLL4; Delta4; Delta-like 4
Gene/Protein ID Q9NR61
Species Human
Exact Sequence Ser27-Pro524
Molecular Weight The protein has a predicted MW of 55.7 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-DLL4 Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human DLL4, His Tag with the EC50 of 0.33μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-DLL4 Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human DLL4, His Tag with the EC50 of 0.33μg/ml determined by ELISA.
The purity of Biotinylated Human DLL4 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human DLL4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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