Product Description |
Recombinant Biotinylated Human DLL4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser27-Pro524. |
Product Background |
Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate. |
Product Category |
Recombinant Protein |
Target |
DLL4 |
Synonyms |
DLL4; MGC126344; notch ligand DLL4; Delta4; Delta-like 4 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ser27-Pro524 |
Molecular Weight |
The protein has a predicted MW of 55.7 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human DLL4, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human DLL4, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). |
|
|
Biotinylated Human DLL4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Biotinylated Human DLL4 is greater than 95% as determined by SEC-HPLC. |
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