Biotinylated Human DLL4 Protein (DLL-HM404B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Biotinylated: yes
Activity: Immobilized Anti-DLL4 Antibody, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human DLL4, His Tag with the EC50 of 0.21ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser27-Pro524

Catalog: DLL-HM404B

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Product Description	Recombinant Biotinylated Human DLL4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser27-Pro524.
Product Background	Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.
Product Category	Recombinant Protein

Target	DLL4
Synonyms	DLL4; MGC126344; notch ligand DLL4; Delta4; Delta-like 4
Gene/Protein ID	Q9NR61
Species	Human
Exact Sequence	Ser27-Pro524
Molecular Weight	The protein has a predicted MW of 55.7 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Anti-DLL4 Antibody, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human DLL4, His Tag with the EC50 of 0.21ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Anti-DLL4 Antibody, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human DLL4, His Tag with the EC50 of 0.21ug/ml determined by ELISA.
	The purity of Biotinylated Human DLL4 is greater than 95% as determined by SEC-HPLC.
	Biotinylated Human DLL4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.