Name: Biotinylated Human DLL4 Protein (DLL-HM404B)
Brand: KactusBio
SKU: DLL-HM404B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human DLL4 Protein (DLL-HM404B)

Immobilized Biotinylated Human DLL4, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test).

Biotinylated Human DLL4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human DLL4 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human DLL4, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ser27-Pro524

Catalog #DLL-HM404B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human DLL4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser27-Pro524.
Product Background	Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.
Product Category	Recombinant Protein / Antibody discovery

Protein	DLL4
Synonyms	DLL-4; MGC126344; notch ligand DLL4; Delta4; Delta-like 4
Accession	Q9NR61
Species	Human
Biotinylated	yes
Amino Acid Range	Ser27-Pro524
Molecular Weight	The protein has a predicted MW of 55.7 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human DLL4, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human DLL4, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test).
	Biotinylated Human DLL4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human DLL4 is greater than 95% as determined by SEC-HPLC.