Biotinylated Human FGF10 Protein (Primary Amine Labeling) (FGF-HE010B)

  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 90% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: The affinity constant of 147.70 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln38-Ser208

Catalog: FGF-HE010B

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Product Description Recombinant Biotinylated Human FGF10 Protein (Primary Amine Labeling) is expressed from E.coli without tag.It contains Gln38-Ser208.

Product Background

 Fibroblast growth factor 10 (FGF10) regulates multiple stages of structural lung morphogenesis, cellular differentiation, and the response to injury. As a driver of lung airway branching morphogenesis, FGF10 signaling defects during development lead to neonatal lung disease. Lung diseases impact patients across the lifespan, from infants in the first minutes of life through the aged population. Congenital abnormalities of lung structure can cause lung disease at birth or make adults more susceptible to chronic disease.

Product Category

 Recombinant Protein

Target

 FGF10
Synonyms FGF-10; KGF2

Gene/Protein ID

 O15520

Species

 Human

Exact Sequence

 Gln38-Ser208
Molecular Weight The protein has a predicted MW of 19.3 kDa same as Tris-Bis PAGE result.

Activity

 The affinity constant of 147.70 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

 No Tag

Express System

 E.coli
Purity > 90% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 20mM Tris,150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human FGF10, No tag immobilized on CM5 Chip can bind Human FGFR2 alpha IIIb, His tag with an affinity constant of 147.70 nM as determined in SPR assay (Biacore T200) (QC Test).
Serial dilutions of Anti-FGFR2 alpha (IIIb) Antibody were added into Human FGFR2 alpha (IIIb) , His Tag : Biotinylated Human FGF10, No Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.7ng/ml.
The purity of Biotinylated Human FGF10 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human FGF10 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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