Name: Biotinylated Human FGF10 Protein (Primary Amine Labeling) (FGF-HE010B)
Brand: KactusBio
SKU: FGF-HE010B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human FGF10 Protein (Primary Amine Labeling) (FGF-HE010B)

Biotinylated Human FGF10, No tag immobilized on CM5 Chip can bind Human FGFR2 alpha IIIb, His tag with an affinity constant of 147.70 nM as determined in SPR assay (Biacore T200) (QC Test).

Biotinylated Human FGF10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Serial dilutions of Anti-FGFR2 alpha (IIIb) Antibody were added into Human FGFR2 alpha (IIIb) , His Tag : Biotinylated Human FGF10, No Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.7ng/ml.

The purity of Biotinylated Human FGF10 is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 147.70 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln38-Ser208

Catalog #FGF-HE010B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human FGF10 Protein (Primary Amine Labeling) (FGF-HE010B) Summary

Product Description	Recombinant Biotinylated Human FGF10 Protein (Primary Amine Labeling) is expressed from E.coli without tag.It contains Gln38-Ser208.
Product Background	Fibroblast growth factor 10 (FGF10) regulates multiple stages of structural lung morphogenesis, cellular differentiation, and the response to injury. As a driver of lung airway branching morphogenesis, FGF10 signaling defects during development lead to neonatal lung disease. Lung diseases impact patients across the lifespan, from infants in the first minutes of life through the aged population. Congenital abnormalities of lung structure can cause lung disease at birth or make adults more susceptible to chronic disease.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human FGF10 Protein (Primary Amine Labeling) (FGF-HE010B) Specifications/Details

Protein	FGF10
Synonyms	FGF-10; KGF2
Accession	O15520
Species	Human
Biotinylated	yes
Amino Acid Range	Gln38-Ser208
Molecular Weight	The protein has a predicted MW of 19.3 kDa same as Bis-Tris PAGE result.
Activity	The affinity constant of 147.70 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris,150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Biotinylated Human FGF10, No tag immobilized on CM5 Chip can bind Human FGFR2 alpha IIIb, His tag with an affinity constant of 147.70 nM as determined in SPR assay (Biacore T200) (QC Test).
	Biotinylated Human FGF10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Serial dilutions of Anti-FGFR2 alpha (IIIb) Antibody were added into Human FGFR2 alpha (IIIb) , His Tag : Biotinylated Human FGF10, No Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.7ng/ml.
	The purity of Biotinylated Human FGF10 is greater than 95% as determined by SEC-HPLC.