Biotinylated Human HLA-A*02:01&B2M&TdT (ALYDKTKRIFL) Monomer Protein (

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYDKTKRIFL peptide

Catalog #MHC-HM487B

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$341.00

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Biotinylated Human HLA-A*02:01&B2M&TdT (ALYDKTKRIFL) Monomer Protein (MHC-HM487B) Summary

Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&TdT (ALYDKTKRIFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and ALYDKTKRIFL peptide.
Product Background	The terminal deoxynucleotidyl transferase (TdT) belongs to the X family of DNA polymerases. This unusual polymerase catalyzes the template-independent addition of random nucleotides on 3'-overhangs during V(D)J recombination. The biological function and intrinsic biochemical properties of the TdT have spurred the development of numerous oligonucleotide-based tools and methods, especially if combined with modified nucleoside triphosphates.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

Protein	HLA-A*02:01&B2M&TdT (ALYDKTKRIFL)
Synonyms	DNA nucleotidylexotransferase; terminal deoxynucleotidyl transferase
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&ALYDKTKRIFL
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYDKTKRIFL peptide
Molecular Weight	The protein has a predicted MW of 50.80 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
Product Tag	C-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Biotinylated Human HLA-A*02:01&B2M&TdT (ALYDKTKRIFL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HLA-A*02:01&B2M&TdT (ALYDKTKRIFL) Monomer is greater than 95% as determined by SEC-HPLC.

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