• Express System: E.coli
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human IL-21R, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-21, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Gln32-Ser162
Catalog #IL2-HE421B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Biotinylated Human IL-21 Protein (IL2-HE421B) Summary

Product Description

 Recombinant Biotinylated Human IL-21 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Gln32-Ser162.

Product Background

 Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases.

Biotinylated Human IL-21 Protein (IL2-HE421B) Specifications/Details

Protein
 IL-21
Synonyms
 Interleukin-21; IL-21; Za11; CVID11;IL21;IL 21; Interleukin 21
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gln32-Ser162
Molecular Weight
 The protein has a predicted MW of 18.30 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human IL-21R, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-21, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test).
Product Tag
 N-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20mM NaAc, 500mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in 20mM NaAc (pH 5.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-21R, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-21, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test). Immobilized Human IL-21R, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-21, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test).
Biotinylated Human IL-21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IL-21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...