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Biotinylated Human Kallikrein 3/PSA-Protein (active form) (Primary Amine Labeling) (KLK-HM13AB) Summary
Product Description |
Recombinant Biotinylated Human Kallikrein 3/PSA-Protein (active form) (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-terminus.It contains Ile25-Pro261. |
Product Background |
Kallikrein-related peptidase 3 (KLK3), also known as prostate-specific antigen (PSA), is the most useful biomarker for prostate cancer (PCa). KLK3 is suggested to play a role in regulating cancer growth through anti-angiogenic activity in vivo and in vitro. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human Kallikrein 3/PSA-Protein (active form) (Primary Amine Labeling) (KLK-HM13AB) Specifications/Details
Protein |
Kallikrein 3/PSA |
Synonyms |
PSA; Kallikrein 3; Kallikrein-3; P-30 antigen; Semenogelase; KLK3; APS |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ile25-Pro261 |
Molecular Weight |
The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 31-38 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human Kallikrein 3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Kallikrein 3 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA). The specific activity is >200 pmol/min/ug. |
Product Tag |
C-His |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 50mM Tris, 150mM NaCl, 8% trehalose, 0.05% Brij-35 (pH 7.5). |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human Kallikrein 3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Kallikrein 3 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. |
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Biotinylated Human Kallikrein 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Biotinylated Human Kallikrein 3 (active form), His Tag on Steptavidin-Biosensor can bind Anti-Kallikrein 3 Antibody, hFc Tag with an affinity constant of 0.14 nM as determined in BLI assay. |
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The purity of Biotinylated Human Kallikrein 3 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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