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Biotinylated Human LILRA5/CD85f/ILT11 Protein (LIL-HM4A5B) Summary
Product Description |
Recombinant Biotinylated Human LILRA5/CD85f/ILT11 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly42-Arg268. |
Product Background |
The leukocyte immunoglobulin-like receptors (LILR) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIRs). Human LILRA5, also known as ILT11, LIR-9, and CD85f, consists of a 227 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 10 aa cytoplasmic tail. LILRA5 may play a role in triggering innate immune responses. Does not seem to play a role for any class I MHC antigen recognition. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Biotinylated Human LILRA5/CD85f/ILT11 Protein (LIL-HM4A5B) Specifications/Details
Protein |
LILRA5/CD85f/ILT11 |
Synonyms |
CD85; CD85f; ILT11; ILT-11; ILT11CD85f; LILRA5; LILRB7; LIR9; LIR-9 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly42-Arg268 |
Molecular Weight |
The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Biotinylated Human LILRA5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human LILRA5 is greater than 95% as determined by SEC-HPLC. |
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